Recombinant:Article Title: Structure of the MIWI endoribonuclease bound to pachytene piRNAs from mouse testes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal mouse anti-Y12 Brahms et al.26; Lerner et al.27 Available from lead contact Monoclonal mouse MILI antibody (13E-3) Reuter et al.23 Available from lead contact Polyclonal Rabbit anti-MIWI BTO Reuter et al.4 Available from lead contact β-Actin Antibody Santa Cruz Cat. No. sc-47778 ; RRID: AB_626632 anti-TUBULIN Abcam Cat. No. ab6046 ; RRID: AB_2210370 IRDye® 800CW Donkey anti-Mouse IgG Secondary Antibody Licorbio Cat. No. 926-32212 ; RRID: AB_621847 IRDye® 680RD Goat anti-Rabbit IgG Secondary Antibody Licorbio Cat. No. 926-68071 ; RRID:AB_10956166 Goat anti-Rabbit IgG (H + L) CrossAdsorbed Secondary Antibody, Alexa FluorTM 488 Invitrogen Cat. No. A11008 ; RRID: AB_143165 HRP-conjugated anti-rabbit IgG GE Healthcare Cat. No. NA934; RRID: AB_772206 Chemicals, Peptides, and Recombinant Proteins N,N,N′,N′-Tetramethylethylendiamin Merck Cat. No. 1107320100 40% Acrylamide/Bis Solution Biorad Cat. No. 1610144 Ultra-Pure ProtoGel 30% acrylamide (37.5:1) National Diagnostic Cat. no. EC-890 Amersham Prime Western Blotting Detection Reagent GE Healthcare Cat. no. RPN2232 Sodium deoxycholate Sigma Cat. No. 30968 Trypsin-EDTA (0.25%), phenol red Thermo Fischer Cat. No. 25200056 Collagenase from Clostridium histolyticum Sigma Cat. No. C5138 Turbo DNase Invitogen Cat No. AM2238 Hoechst 33342 Thermo Scientific Cat. No. 62249 Vanadyl ribonucleoside complexes solution Sigma Cat. No. 94742-10ML Tween 20 SIGMA Cat. No. P7949 Phire Green Hot Start II PCR Master Mix Thermo Scientific Cat. No. F126L γ-32P- Adenosine triphosphate Hartmann Analytic Cat. No. FP-301 T4 Polynucleotide Kinase NEB Cat. No. M0201L MethaPhor agarose Lonza Cat. No. 50180 Protein G Sepharose 4 Fast Flow Cytiva Cat. No. 17061801 Strep-Tactin XT resin Cytiva Cat. No. 29401326 Biolock IBA Cat. No. 2-0205-050 PageBlueTM Protein Staining Solution Thermo Fisher Cat. No. 24620 4-Thio-UTP Jena Bioscience Cat. No. NU-1156L Proteinase K Thermo Fisher Cat. No. AM2542 Acid-Phenol:Chloroform, pH 4.5 Thermo Fisher Cat. No. AM9722 Alt-RTM S.p. .. Cas9 Nuclease V3 IDT Cat. No. 1081058 IDTE pH 7.5 (1× TE Solution) IDT Cat. No. 11-05-01-05 PierceTM Protease Inhibitor Tablets, EDTAfree Thermo Fisher Cat. no. A32965 OCT Embedding Matrix for Frozen Section VWR Cat. no. 361603E RiboLock RNase Inhibitor Thermo Fisher Cat. no. EO0381 Chloroform Merck Cat. no. 102445 (Continued on next page) 14 Cell Reports 45, 116804, January 27, 2026
Diagnostic Assay:Article Title: Structure of the MIWI endoribonuclease bound to pachytene piRNAs from mouse testes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal mouse anti-Y12 Brahms et al.26; Lerner et al.27 Available from lead contact Monoclonal mouse MILI antibody (13E-3) Reuter et al.23 Available from lead contact Polyclonal Rabbit anti-MIWI BTO Reuter et al.4 Available from lead contact β-Actin Antibody Santa Cruz Cat. No. sc-47778 ; RRID: AB_626632 anti-TUBULIN Abcam Cat. No. ab6046 ; RRID: AB_2210370 IRDye® 800CW Donkey anti-Mouse IgG Secondary Antibody Licorbio Cat. No. 926-32212 ; RRID: AB_621847 IRDye® 680RD Goat anti-Rabbit IgG Secondary Antibody Licorbio Cat. No. 926-68071 ; RRID:AB_10956166 Goat anti-Rabbit IgG (H + L) CrossAdsorbed Secondary Antibody, Alexa FluorTM 488 Invitrogen Cat. No. A11008 ; RRID: AB_143165 HRP-conjugated anti-rabbit IgG GE Healthcare Cat. No. NA934; RRID: AB_772206 Chemicals, Peptides, and Recombinant Proteins N,N,N′,N′-Tetramethylethylendiamin Merck Cat. No. 1107320100 40% Acrylamide/Bis Solution Biorad Cat. No. 1610144 Ultra-Pure ProtoGel 30% acrylamide (37.5:1) National Diagnostic Cat. no. EC-890 Amersham Prime Western Blotting Detection Reagent GE Healthcare Cat. no. RPN2232 Sodium deoxycholate Sigma Cat. No. 30968 Trypsin-EDTA (0.25%), phenol red Thermo Fischer Cat. No. 25200056 Collagenase from Clostridium histolyticum Sigma Cat. No. C5138 Turbo DNase Invitogen Cat No. AM2238 Hoechst 33342 Thermo Scientific Cat. No. 62249 Vanadyl ribonucleoside complexes solution Sigma Cat. No. 94742-10ML Tween 20 SIGMA Cat. No. P7949 Phire Green Hot Start II PCR Master Mix Thermo Scientific Cat. No. F126L γ-32P- Adenosine triphosphate Hartmann Analytic Cat. No. FP-301 T4 Polynucleotide Kinase NEB Cat. No. M0201L MethaPhor agarose Lonza Cat. No. 50180 Protein G Sepharose 4 Fast Flow Cytiva Cat. No. 17061801 Strep-Tactin XT resin Cytiva Cat. No. 29401326 Biolock IBA Cat. No. 2-0205-050 PageBlueTM Protein Staining Solution Thermo Fisher Cat. No. 24620 4-Thio-UTP Jena Bioscience Cat. No. NU-1156L Proteinase K Thermo Fisher Cat. No. AM2542 Acid-Phenol:Chloroform, pH 4.5 Thermo Fisher Cat. No. AM9722 Alt-RTM S.p. .. Cas9 Nuclease V3 IDT Cat. No. 1081058 IDTE pH 7.5 (1× TE Solution) IDT Cat. No. 11-05-01-05 PierceTM Protease Inhibitor Tablets, EDTAfree Thermo Fisher Cat. no. A32965 OCT Embedding Matrix for Frozen Section VWR Cat. no. 361603E RiboLock RNase Inhibitor Thermo Fisher Cat. no. EO0381 Chloroform Merck Cat. no. 102445 (Continued on next page) 14 Cell Reports 45, 116804, January 27, 2026
Western Blot:Article Title: Structure of the MIWI endoribonuclease bound to pachytene piRNAs from mouse testes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal mouse anti-Y12 Brahms et al.26; Lerner et al.27 Available from lead contact Monoclonal mouse MILI antibody (13E-3) Reuter et al.23 Available from lead contact Polyclonal Rabbit anti-MIWI BTO Reuter et al.4 Available from lead contact β-Actin Antibody Santa Cruz Cat. No. sc-47778 ; RRID: AB_626632 anti-TUBULIN Abcam Cat. No. ab6046 ; RRID: AB_2210370 IRDye® 800CW Donkey anti-Mouse IgG Secondary Antibody Licorbio Cat. No. 926-32212 ; RRID: AB_621847 IRDye® 680RD Goat anti-Rabbit IgG Secondary Antibody Licorbio Cat. No. 926-68071 ; RRID:AB_10956166 Goat anti-Rabbit IgG (H + L) CrossAdsorbed Secondary Antibody, Alexa FluorTM 488 Invitrogen Cat. No. A11008 ; RRID: AB_143165 HRP-conjugated anti-rabbit IgG GE Healthcare Cat. No. NA934; RRID: AB_772206 Chemicals, Peptides, and Recombinant Proteins N,N,N′,N′-Tetramethylethylendiamin Merck Cat. No. 1107320100 40% Acrylamide/Bis Solution Biorad Cat. No. 1610144 Ultra-Pure ProtoGel 30% acrylamide (37.5:1) National Diagnostic Cat. no. EC-890 Amersham Prime Western Blotting Detection Reagent GE Healthcare Cat. no. RPN2232 Sodium deoxycholate Sigma Cat. No. 30968 Trypsin-EDTA (0.25%), phenol red Thermo Fischer Cat. No. 25200056 Collagenase from Clostridium histolyticum Sigma Cat. No. C5138 Turbo DNase Invitogen Cat No. AM2238 Hoechst 33342 Thermo Scientific Cat. No. 62249 Vanadyl ribonucleoside complexes solution Sigma Cat. No. 94742-10ML Tween 20 SIGMA Cat. No. P7949 Phire Green Hot Start II PCR Master Mix Thermo Scientific Cat. No. F126L γ-32P- Adenosine triphosphate Hartmann Analytic Cat. No. FP-301 T4 Polynucleotide Kinase NEB Cat. No. M0201L MethaPhor agarose Lonza Cat. No. 50180 Protein G Sepharose 4 Fast Flow Cytiva Cat. No. 17061801 Strep-Tactin XT resin Cytiva Cat. No. 29401326 Biolock IBA Cat. No. 2-0205-050 PageBlueTM Protein Staining Solution Thermo Fisher Cat. No. 24620 4-Thio-UTP Jena Bioscience Cat. No. NU-1156L Proteinase K Thermo Fisher Cat. No. AM2542 Acid-Phenol:Chloroform, pH 4.5 Thermo Fisher Cat. No. AM9722 Alt-RTM S.p. .. Cas9 Nuclease V3 IDT Cat. No. 1081058 IDTE pH 7.5 (1× TE Solution) IDT Cat. No. 11-05-01-05 PierceTM Protease Inhibitor Tablets, EDTAfree Thermo Fisher Cat. no. A32965 OCT Embedding Matrix for Frozen Section VWR Cat. no. 361603E RiboLock RNase Inhibitor Thermo Fisher Cat. no. EO0381 Chloroform Merck Cat. no. 102445 (Continued on next page) 14 Cell Reports 45, 116804, January 27, 2026
Polymerase Chain Reaction:Article Title: Structure of the MIWI endoribonuclease bound to pachytene piRNAs from mouse testes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal mouse anti-Y12 Brahms et al.26; Lerner et al.27 Available from lead contact Monoclonal mouse MILI antibody (13E-3) Reuter et al.23 Available from lead contact Polyclonal Rabbit anti-MIWI BTO Reuter et al.4 Available from lead contact β-Actin Antibody Santa Cruz Cat. No. sc-47778 ; RRID: AB_626632 anti-TUBULIN Abcam Cat. No. ab6046 ; RRID: AB_2210370 IRDye® 800CW Donkey anti-Mouse IgG Secondary Antibody Licorbio Cat. No. 926-32212 ; RRID: AB_621847 IRDye® 680RD Goat anti-Rabbit IgG Secondary Antibody Licorbio Cat. No. 926-68071 ; RRID:AB_10956166 Goat anti-Rabbit IgG (H + L) CrossAdsorbed Secondary Antibody, Alexa FluorTM 488 Invitrogen Cat. No. A11008 ; RRID: AB_143165 HRP-conjugated anti-rabbit IgG GE Healthcare Cat. No. NA934; RRID: AB_772206 Chemicals, Peptides, and Recombinant Proteins N,N,N′,N′-Tetramethylethylendiamin Merck Cat. No. 1107320100 40% Acrylamide/Bis Solution Biorad Cat. No. 1610144 Ultra-Pure ProtoGel 30% acrylamide (37.5:1) National Diagnostic Cat. no. EC-890 Amersham Prime Western Blotting Detection Reagent GE Healthcare Cat. no. RPN2232 Sodium deoxycholate Sigma Cat. No. 30968 Trypsin-EDTA (0.25%), phenol red Thermo Fischer Cat. No. 25200056 Collagenase from Clostridium histolyticum Sigma Cat. No. C5138 Turbo DNase Invitogen Cat No. AM2238 Hoechst 33342 Thermo Scientific Cat. No. 62249 Vanadyl ribonucleoside complexes solution Sigma Cat. No. 94742-10ML Tween 20 SIGMA Cat. No. P7949 Phire Green Hot Start II PCR Master Mix Thermo Scientific Cat. No. F126L γ-32P- Adenosine triphosphate Hartmann Analytic Cat. No. FP-301 T4 Polynucleotide Kinase NEB Cat. No. M0201L MethaPhor agarose Lonza Cat. No. 50180 Protein G Sepharose 4 Fast Flow Cytiva Cat. No. 17061801 Strep-Tactin XT resin Cytiva Cat. No. 29401326 Biolock IBA Cat. No. 2-0205-050 PageBlueTM Protein Staining Solution Thermo Fisher Cat. No. 24620 4-Thio-UTP Jena Bioscience Cat. No. NU-1156L Proteinase K Thermo Fisher Cat. No. AM2542 Acid-Phenol:Chloroform, pH 4.5 Thermo Fisher Cat. No. AM9722 Alt-RTM S.p. .. Cas9 Nuclease V3 IDT Cat. No. 1081058 IDTE pH 7.5 (1× TE Solution) IDT Cat. No. 11-05-01-05 PierceTM Protease Inhibitor Tablets, EDTAfree Thermo Fisher Cat. no. A32965 OCT Embedding Matrix for Frozen Section VWR Cat. no. 361603E RiboLock RNase Inhibitor Thermo Fisher Cat. no. EO0381 Chloroform Merck Cat. no. 102445 (Continued on next page) 14 Cell Reports 45, 116804, January 27, 2026
Staining:Article Title: Structure of the MIWI endoribonuclease bound to pachytene piRNAs from mouse testes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal mouse anti-Y12 Brahms et al.26; Lerner et al.27 Available from lead contact Monoclonal mouse MILI antibody (13E-3) Reuter et al.23 Available from lead contact Polyclonal Rabbit anti-MIWI BTO Reuter et al.4 Available from lead contact β-Actin Antibody Santa Cruz Cat. No. sc-47778 ; RRID: AB_626632 anti-TUBULIN Abcam Cat. No. ab6046 ; RRID: AB_2210370 IRDye® 800CW Donkey anti-Mouse IgG Secondary Antibody Licorbio Cat. No. 926-32212 ; RRID: AB_621847 IRDye® 680RD Goat anti-Rabbit IgG Secondary Antibody Licorbio Cat. No. 926-68071 ; RRID:AB_10956166 Goat anti-Rabbit IgG (H + L) CrossAdsorbed Secondary Antibody, Alexa FluorTM 488 Invitrogen Cat. No. A11008 ; RRID: AB_143165 HRP-conjugated anti-rabbit IgG GE Healthcare Cat. No. NA934; RRID: AB_772206 Chemicals, Peptides, and Recombinant Proteins N,N,N′,N′-Tetramethylethylendiamin Merck Cat. No. 1107320100 40% Acrylamide/Bis Solution Biorad Cat. No. 1610144 Ultra-Pure ProtoGel 30% acrylamide (37.5:1) National Diagnostic Cat. no. EC-890 Amersham Prime Western Blotting Detection Reagent GE Healthcare Cat. no. RPN2232 Sodium deoxycholate Sigma Cat. No. 30968 Trypsin-EDTA (0.25%), phenol red Thermo Fischer Cat. No. 25200056 Collagenase from Clostridium histolyticum Sigma Cat. No. C5138 Turbo DNase Invitogen Cat No. AM2238 Hoechst 33342 Thermo Scientific Cat. No. 62249 Vanadyl ribonucleoside complexes solution Sigma Cat. No. 94742-10ML Tween 20 SIGMA Cat. No. P7949 Phire Green Hot Start II PCR Master Mix Thermo Scientific Cat. No. F126L γ-32P- Adenosine triphosphate Hartmann Analytic Cat. No. FP-301 T4 Polynucleotide Kinase NEB Cat. No. M0201L MethaPhor agarose Lonza Cat. No. 50180 Protein G Sepharose 4 Fast Flow Cytiva Cat. No. 17061801 Strep-Tactin XT resin Cytiva Cat. No. 29401326 Biolock IBA Cat. No. 2-0205-050 PageBlueTM Protein Staining Solution Thermo Fisher Cat. No. 24620 4-Thio-UTP Jena Bioscience Cat. No. NU-1156L Proteinase K Thermo Fisher Cat. No. AM2542 Acid-Phenol:Chloroform, pH 4.5 Thermo Fisher Cat. No. AM9722 Alt-RTM S.p. .. Cas9 Nuclease V3 IDT Cat. No. 1081058 IDTE pH 7.5 (1× TE Solution) IDT Cat. No. 11-05-01-05 PierceTM Protease Inhibitor Tablets, EDTAfree Thermo Fisher Cat. no. A32965 OCT Embedding Matrix for Frozen Section VWR Cat. no. 361603E RiboLock RNase Inhibitor Thermo Fisher Cat. no. EO0381 Chloroform Merck Cat. no. 102445 (Continued on next page) 14 Cell Reports 45, 116804, January 27, 2026
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